Comments (3)
The exact pipelines used for all of image processing can be found here. It is the same kind of correction in those papers, most recently described here.
I believe that all sources performed the flat field illumination correction before segmentation and measurements.
Yes, the raw images were divided by the .npy correction functions.
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Hi @dpaysan, thanks for your interest in our dataset. I am tagging @bethac07 and @ErinWeisbart who can answer your questions about illumination correction.
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Thanks so much! That helps a lot. Seems like the illumination pipeline is largely the same with the exception of the additional resizing steps for the optimized protocol and the adjusted Median filter size (20 in the optimized vs 200 before).
Excited to see this tremendous effort and data set to continue to develop!
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Related Issues (20)
- How to obtain ORF features HOT 4
- Weird batch effects in source_1
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