Comments (2)
Hello,
as I recall, the sequel outputs quality values '!' which are equal to 0. Could you please verify this with your fastq file? If that is the case, Racon entered an infinite loop in graph traversal as all edges of the graph have weight 0. You can circumvent this by replacing all '!' with '"' in your fastq file. The newer version of Racon will have a check for this case built in.
Best regards,
Robert
from racon.
Hi Robert,
Thank you for your message. You are correct that the quality scores for the fastq are all '!'. I converted these to '"' as suggested and now racon does seem to be processing through the draft. I will close this issue.
from racon.
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