Issue with accessing data in .fcs files about flowcytometrytools HOT 6 CLOSED

Could you attach your FCS file, so I could debug?

Could you also confirm that you're using using python 2.7 rather than python >3?

At the command terminal you can type the following:

python --version

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I'm running Python 2.7.10 64-bit on a mac (os x Yosemite).

I've attached a zipped file below, since it didn't support to attach .fcs files. Hope it doesn't mess with your AV too much.

Regards
JLautin
LMO_bacteria.fcs.zip

from flowcytometrytools.

Thanks! I will take a look at this over the weekend.

On Thu, Dec 17, 2015 at 9:28 AM, JLautin [email protected] wrote:

I'm running Python 2.7.10 64-bit on a mac (os x Yosemite).

I've attached a zipped file below, since it didn't support to attach .fcs
files. Hope it doesn't mess with your AV too much.

Regards
JLautin
LMO_bacteria.fcs.zip
https://github.com/eyurtsev/FlowCytometryTools/files/65467/LMO_bacteria.fcs.zip

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#9 (comment)
.

Eugene Yurtsev

Personal Website: http://eyurtsev.mit.edu

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Looks like this is caused by an issue with numpy numpy/numpy#2407

I'll try and release a fix within the next few days

If you need a fix quicker than that you can try and do the following:

1. locate the directory for the fcsparser package

import fcsparser
fcsparser.__path__

2. go to that directory and open api.py

3. Make the following change in the code:

@@ -353,7 +353,8 @@ class FCSParser(object):
             # values saved in mixed data formats
             dtype = ','.join(par_numeric_type_list)
             data = numpy.fromfile(file_handle, dtype=dtype, count=num_events)
-            data.dtype.names = self.get_channel_names()
+            names = self.get_channel_names()
+            data.dtype.names = [name.encode('ascii', errors='ignore') for name in names]
         else:
             # values saved in a single data format
             dtype = par_numeric_type_list[0]

where a (-) is remove, and a (+) is add

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Fixed in this commit: eyurtsev/fcsparser@398b6b0

Please install the newest version of fcsparser (and FlowCytometryTools)

pip install fcsparser==0.1.2
pip install FlowCytometryTools==0.4.5

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Thank you for solving it!

/JLautin

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