Comments (1)
Sorry for the lack of documentation. See slides 221-225 here. When you use the coarse-grain/fine-grain decomposition approach, there is a first quick sequence decomposition to find good cuts, then a refinement around these cuts, to find even better cuts. The cut_spread_radius
is how many nucleotides away the better cuts can be from the good cuts.
I'm not 100% sure how this could impact circular sequence decomposition (but I understand that it could), it may depend on how you define the circularity. It's difficult to say without a minimal failing example.
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Related Issues (12)
- dealing with circular DNA HOT 18
- Example of using PartsLibrary with Gibson assembly method HOT 2
- File Not Found Error during assembly_report execution HOT 2
- What to do when Gibson assembly Station is refused : Apologies to put a routine use question as an issue HOT 10
- Contact Zulko HOT 7
- verify_constraints performance HOT 3
- SBOL => Automation MoClo Assembly tool => Optimal assembly report HOT 2
- Does the PartsLibrary work for circular sequences? HOT 3
- Q: Any specific reason why PcrExtractionStation not using the PartsLibrary? HOT 1
- Bug: Max length initialisation HOT 2
- Bug: When quote.assembly_plan is None
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