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dtm2451 avatar dtm2451 commented on June 1, 2024

Hmmm might help to have an even simpler code example that I could follow along myself, i.e. without needing your s.integrated or gene_ID_to_name.csv. But I think that your issue is that labels_row should be given labels in the pre-clustering order, not post-clustering. Try giving them in the same order as your genes.

(For your ggplot code attempt, pheatmap doesn't output a pure ggplot object unfortunately so that wouldn't work.)

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dtm2451 avatar dtm2451 commented on June 1, 2024

Assuming that this issue is resolved, but feel free to reopen if not!

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rahulnutron avatar rahulnutron commented on June 1, 2024

Hmmm might help to have an even simpler code example that I could follow along myself, i.e. without needing your s.integrated or gene_ID_to_name.csv. But I think that your issue is that labels_row should be given labels in the pre-clustering order, not post-clustering. Try giving them in the same order as your genes.

(For your ggplot code attempt, pheatmap doesn't output a pure ggplot object unfortunately so that wouldn't work.)

Thanks for your reply! however the suggestion to give them in the same order also didn't worked. But I managed to do that using this code

rownames(data_avg@assays$RNA@data)<-paste0(dicn_names[rownames(data_avg@assays$RNA@data)])
dittoHeatmap(data_avg,treeheight_row = 0,annot.by = c( "CellType","Condition"),order.by = c( "CellType","Condition"))

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