Comments (3)
Hello, The reason that the expression family is not binomial is that the differential expression is run on an aggregated dataset which should move into the normal approximation. As to why your data is not giving any DA peaks, it is hard to say without more information. Do you have a well fit pseudotime curve? Are the data very homogenous? Perhaps I could help with further information.
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I'm going to close, reopen if you have further questions
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