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quinamatics avatar quinamatics commented on September 27, 2024

Additionally, when I force install monocle3 again, the block seems to complete but I'm not sure if the other building/installing processes are even running. The screenshot of this is below

Screen Shot 2020-11-12 at 8 07 25 PM

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hpliner avatar hpliner commented on September 27, 2024

Hello, from your code it looks like somehow Cicero for monocle2 is being loaded. Can you post your sessionInfo()?

After reinstalling the various packages, are you restarting your R session? R does not reload packages that are already loaded even if they have been reinstalled, so if you haven't restarted R you will keep the same packages.

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quinamatics avatar quinamatics commented on September 27, 2024

Here's my session info as well as the log for input_cds and the error (which is slighly different, but input_cds is still not correct). This is also after I restarted my R session (I just restarted the notebook kernel, if this isn't equivalent please let me know).

Screen Shot 2020-12-08 at 12 23 42 PM

Screen Shot 2020-12-08 at 12 25 17 PM

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hpliner avatar hpliner commented on September 27, 2024

input_cds is correct. The correct class with monocle3 is cell_data_set

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hpliner avatar hpliner commented on September 27, 2024

The error you posted is related to the fact that the UMAP slot is empty. Have you calculated a UMAP?

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quinamatics avatar quinamatics commented on September 27, 2024

Hi, I've gotten past that error. However I now have this error. Upon searching in past examples it seems like this exact issue hasn't been brought up yet. I've also posted screenshots of my input_cds and umap coordinates

Screen Shot 2020-12-21 at 7 43 40 PM

Screen Shot 2020-12-21 at 7 44 31 PM

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quinamatics avatar quinamatics commented on September 27, 2024

Also, I saw the error here: cole-trapnell-lab/monocle3#291, but I've already included the fix in my code. Here is a screenshot of how I work with the input_cds, also there is a warning about using a standard svd. Is that the reason behind the bug?
Screen Shot 2020-12-21 at 8 21 01 PM

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hpliner avatar hpliner commented on September 27, 2024

Hello,
You're running into this error because you're attempting to make a cicero cds with only 80 cells - with a k value of 50, it's not possible to make sufficient aggregated cell groups. Internally such a small matrix gets converted to a different type which throws the error.

While you could get past the error by lower the k value, Cicero is designed to be used for many more cells (maybe 500), so I would not expect robust results with so few cells.

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