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100daysofcloudideas

The purpose of this repo is to provide a list of micro-projects to help people with their Cloud Journey.

100daysofcontainersandorchestration

Your go-to open source repo to learn containers (Docker, Podman, etc.) and Orchestration (Kubernetes, ECS, etc.) from start to finish.

100daysofkubernetes

100DaysOfKubernetes book

30-days-of-javascript

30 days of JavaScript programming challenge is a step by step guide to learn JavaScript programming language in 30 days. This challenge may take up to 100 days, please just follow your own pace.

30-days-of-python

30 days of Python programming challenge is a step by step guide to learn the Python programming language in 30 days. This challenge may take up to 100 days, follow your own pace.

30-days-of-react

30 Days of React challenge is a step by step guide to learn React in 30 days. It requires HTML, CSS, and JavaScript knowledge. You should be comfortable with JavaScript before you start to React. If you are not comfortable with JavaScript check out 30DaysOfJavaScript. This is a continuation of 30 Days Of JS. This challenge may take up to 100 days, follow your own pace.

7-day-youtube-starterkit

Kickstart your cloud YouTube journey in 7 days.

70daysofservicemesh

Inspired by Michael Cades' #90DaysOfDevOps and his ask for me to participate in his 2023 iteration to discuss 7 Days of Service Mesh, I decided to create a #70DaysOfServiceMesh.

90daysofdevops

This repository is my documenting repository for learning the world of DevOps. I started this journey on the 1st January 2022 and I plan to run to March 31st for a complete 90-day romp on spending an hour a day including weekends to get a foundational knowledge across a lot of different areas that make up DevOps.

algorithm-solutions

REPO containing code solutions for algorithm problems, solved in Go & Python to solidify language skills

alnoda-workspaces

Work directly inside docker containers

alwayson

AlwaysOn provides a design methodology and approach to building highly-reliable applications on Microsoft Azure for mission-critical workloads.

angular

One framework. Mobile & desktop.

ansible-for-devops

Ansible for DevOps examples.

app-ideas

A Collection of application ideas which can be used to improve your coding skills.

aquasec_appscope

assemblies-of-putative-sars-cov2-spike-encoding-mrna-sequences-for-vaccines-bnt-162b2-and-mrna-1273

RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.

assessment-questions

automated_app_scope_creation

awesome-actions

A curated list of awesome actions to use on GitHub

awesome-aws-amplify

Curated list of AWS Amplify Resources

awesome-azure-architecture

AWESOME-Azure-Architecture

awesome-bash

A curated list of delightful Bash scripts and resources.

awesome-chainlink

awesome-cloud-foundry

A curated list of Cloud Foundry open-source projects, tools, distributions, talks, and tutorials(videos and blog post)

awesome-cloudnative-trainings

Awesome Trainings from Cloud Native Computing Foundation Projects and Kubernetes related software

awesome-cybersecurity-blueteam

:computer:🛡️ A curated collection of awesome resources, tools, and other shiny things for cybersecurity blue teams.

awesome-design-systems

💅🏻 ⚒ A collection of awesome design systems

awesome-distributed-systems

A curated list to learn about distributed systems

awesome-go

A curated list of awesome Go frameworks, libraries and software