Comments (2)
Greetings @georgysemenov! Thanks very much for raising this issue. The second argument to gtf2revcom must actually be the length of the full chromosome (full reference sequence; FASTA), not the length of any one gene. For example, if the reference sequence is 13 Mbp, you'd use 13000000. Let me know if that makes sense!
Chase
from snpgenie.
Hi Chase! Thank you so much for your help. This was the issue indeed, now it is working as it supposed
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