Comments (5)
I believe this relates to using the subject flag to specify a fasta file to search rather than using makeblastdb and creating a binary database.
I've meant for a while to make this change in cablastp-search (I already made it in cablastp-deltasearch and cablastp-psisearch). I'll try to push a fix tomorrow.
On Nov 14, 2013, at 5:07 PM, nsarode [email protected] wrote:
Here is another one for ya.
Not sure if the real error is "signal: bus error (core dumped)" or "stderr: num_threads' is currently ignored when 'subject' is specified."
Blasting query on coarse database...
blastp -db /blast_databases/cablastp_database/capblastp-cogs_db_aa/blastdb-coarse -num_threads 9 -outfmt 5 -dbsize 47558392
Decompressing blast hits...
Blasting query on fine database...
blastp -subject /tmp/cablastp-fine-fasta604138705 -dbsize 47558392 -num_threads 9 -max_target_seqs 10 -out /blastoutput/stn_ALL_prodigal.prot_2.blast -outfmt 6 qseqid sseqid pident qcovs length mismatch qstart qend sstart send evalue bitscore
Error blasting fine database: Error running 'blastp -subject /tmp/cablastp-fine-fasta604138705 -dbsize 47558392 -num_threads 9 -max_target_seqs 10 -out /blastoutput/stn_ALL_prodigal.prot_2.blast -outfmt 6 qseqid sseqid pident qcovs length mismatch qstart qend sstart send evalue bitscore': 'signal: bus error (core dumped)'.stderr:
'num_threads' is currently ignored when 'subject' is specified.—
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Hi Noah,
Could you confirm if you did manage to make this change ?
Thanks
Neha
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I haven't yet, but that's actually unrelated to the issue you were having, which is why I sidelined it. The issue is one of relative paths, which I believe Andrew (BurntSushi) figured out. We will try to get both of these taken care of soon.
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Thanks for letting me know.
The relative path issue that you mentioned, is it the one where I need to run the blastp search from the database directory ? Because I was doing it this time when I got the same error. But if you are talking about something else, then I will just wait for the update.
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I have pushed what hopefully fixes the 'subject' issue for you. This does not yet solve the need to run the search from the database directory.
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