Comments (11)
Try it again. There wasn't 3.5.1 in docker hub. I just added it.
from somaticseq.
Hi,
it starts now, but eventually fails. I am attaching the output in a file. The error message is
FATAL: Failed to initialize runtime engine: engine "" is not found
Any ideas ?
error.log
Thanks
from somaticseq.
Hi,
I have an update. I processed three samples. The "FATAL: Failed to initialize runtime engine: engine "" is not found" error is only for the first sample. The other two samples are ok. Can you think of any reason for this ?
The other callers, with the exception of vardict2 (I opened another issue for it), work.
Thanks
from somaticseq.
What was the command you've used?
from somaticseq.
Hi,
this error is probably caused by causes external to your code. Possibly, the fact that Singularity defaults to the HOME dir for cache and this may cause issues because of limited quota. After setting the SINGULARITY_CACHEDIR var to a different path it seems ok. If this is indeed the case, I will close this issue.
Thanks for looking into it!
Best
from somaticseq.
Hi,
I am still having an issue. It seems that as soon as one is solved, a new issue comes up....
I am attaching the output from one of the tools. It is the same as for the others. This seem to be happening for Mutect2, Strelka, VarDict, SomaticSniper and JointSNVMix2.
out.log
Can you please look into it and tell me what you think ?
Thanks
from somaticseq.
Did the singularity tasks run despite the warning, or did it stop running?
I'm no expert in singularities. It seems to be complaining about mounting /home/user in your local environment to /home/user in singularities, because somehow the container already has /home/user/project, either because it was mounted already or reasons that I don't know.
from somaticseq.
Thanks for the prompt reply!!
The ones I pointed out stop running. The warning you are referring to is present also in the ones that complete, and I do not think it is a major issue.
In the file I sent you there is a "panic" line after "copying blob". What do you think ?
from somaticseq.
It looks similar to this issue: apptainer/singularity#5329, that may be fixed with this answer apptainer/singularity#5329 (comment).
from somaticseq.
thanks!!
I am testing vardict and, so far, it is not stopping. I am really grateful for your help.
from somaticseq.
It seems ok now.
Thanks!
from somaticseq.
Related Issues (20)
- Special setting for b37? HOT 14
- Question about simulating somatic mutations HOT 7
- Pretrained Classifier HOT 3
- Docker issue with latest version HOT 1
- SEQC2: Some high confidence SNVs and INDELs in VCF are outside of regions defined by High-Confidence_Regions_v1.2.bed HOT 2
- Somaticseq makeSomaticScripts.py running and output issues HOT 8
- Slow RNA variant calling HOT 8
- Question for the paper on establishing the reference call set HOT 3
- Where are the 10x Genomics single-cell copy number variation (CNV) analysis results? HOT 7
- Ground Truths required for training HOT 1
- somaticseq failing for same command it had previously successfully run HOT 11
- Applying internal filters to outputs before running SomaticSeq HOT 1
- Dockerized alignment workflow does not work with multiple input files HOT 5
- Error when running makeSomaticScripts with multiple threads HOT 3
- Output allele of the normal sample HOT 2
- UnboundLocalError: cannot access local variable 'normal_name' where it is not associated with a value HOT 10
- how to obtain all variants where the "FILTER" column is not labeled as "PASS" HOT 1
- Are multi-nucleotide and complex variants ignored? HOT 2
- Error when running FFPE training data from SEQ2C HOT 5
- AI consensus calling error on WGS samples HOT 9
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