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dfornika avatar dfornika commented on July 19, 2024

Sorry but I'm not able to reproduce this. When I run the pipeline I get these headers in the QUAST output:

Assembly
# contigs (>= 0 bp)
# contigs (>= 1000 bp)
# contigs (>= 5000 bp)
# contigs (>= 10000 bp)
# contigs (>= 25000 bp)
# contigs (>= 50000 bp)
Total length (>= 0 bp)
Total length (>= 1000 bp)
Total length (>= 5000 bp)
Total length (>= 10000 bp)
Total length (>= 25000 bp)
Total length (>= 50000 bp)
# contigs
Largest contig
Total length
N50
N75
L50
L75
# N's per 100 kbp

The current report parsing script isn't that intelligent though. I may be able to update it to support any N/L metric, just using a few simple regexes.

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dfornika avatar dfornika commented on July 19, 2024

Ah, I think I may see what the issue is. This pipeline uses QUAST v5.0.2:

Other versions are not supported.

This pipeline is mostly intended for internal use at BCCDC-PHL. Of course you're welcome to use it if you find it useful. But in terms of supporting outside systems and environments we can't offer much.

If you're still interested in running this pipeline I'd suggest using the flags: -profile conda --cache ~/.conda/envs. That will build the conda env based on the environment.yml files in the repo, and the scripts should be consistent with the software versions in those environments.

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