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mosuma_tools's Introduction

MoSuMa_tools

A perl script pipeline for the compilation of Molecular SuperMatrices.

NB: for reasons not understood or resolved, treecleaner.pl can sometimes act unpredictable; this is more common when OTU count is very high. I have not understood why, and I am afraid I don't have the time to look into it and fix it. But please feel free to fork and pull-request :) thanks

Currently, this pipeline deals with two input files: a list of genes (or other sequence data) as BLAST targets, and an assembled transcriptome in which to carry out the BLAST search. Data can be in amino acids or nucleotides. Outputs are aligned matrices for each sequence, ready for concatenation into a supermatrix, gene-tree analysis, or anything else really. The treecleaner.pl script will assess a tree created by phyml for long branches, and remove the seqeunce producing the long branch from the gene matrix.

Please post all bugs or other issues in the issues section of the github page (https://github.com/altanner/MoSuMa_tools/) or contact the repo owner (altanner@github) . Thanks :)

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mosuma_tools's Issues

extract_blast.pl doesn't always select the sequence, due to long numbers of the sequence line

Symptom: the selected_blast *.sel files contain numbers when they should contain sequence information. As far as I can tell, this problem will be more likely with genomic data than transcriptomic, but could effect either.

Diagnosis: During the extract_blast step, the regex/match is searching for a certain number of characters in to start extracting the sequence. It does not match in splitting on spaces, because if if the sequence position changes in number of digits, for example 999 to 1000, the space disappears and the regex splits on the wrong space. This is actually a little tricky to fix, but I know how to do it.

extract blast option for number of high hits needs adding

treecleaner works best with a single high hit being taken. some people might want to expand the range of

  1. number of high hits taken
  2. the width of high hit threshold. at the moment it is 3 order of magnitude from highest hit (unless it is 0.0, in which case all 0.0 hits are taken.)

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