Comments (4)
I'm going to put this in the current Epic we have going just so it gets discussed alongside those issues.
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A note that we may also want to update alevin-fry reading code to use fishpond::loadFry()
, which will simplify some of our code, and can allow us to keep multiple assays, should we so desire: I might propose counts
(U + S + A), spliced
(S + A), and unspliced
(U); This would allow the greatest flexibility for downstream users, even enabling RNA velocity if people want to go that route.
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In looking into this, it appears that Space Ranger does not use introns automatically, and indeed that may not be possible, as I can't find an option in the docs. For Cell Ranger, the option is --include-introns
, but that doesn't seem to exist in Space Ranger. So rerunning Space Ranger files may not be needed.
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Closing this in favor of #229
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Related Issues (20)
- Include instructions for specifying `merge_run_ids` when merging projects in external instructions
- Skip creation of merged objects HOT 1
- Fix column name typos HOT 4
- Future idea: Create merged objects for projects with multiplexed libraries containing all non-multiplexed single-cell libraries
- Prepare for scpca-nf release v0.7.3
- Make sure CellAssign is skipped for any objects with just 1 cell HOT 1
- [BUG] Age in sample_metadata is inconsistently typed HOT 3
- Discussion: Rename AnnData objects with .h5ad extension HOT 6
- [BUG] Account for grabbing estimated demux cell counts for libraries with no genetic demultiplexing HOT 1
- `project_celltype_metafile` parameter is missing from scpca-nf schema
- Test workflow with Bioc3.19 images HOT 1
- Use more specialized docker images for processes HOT 2
- Prepare for scpca-nf release `v0.8.1`
- Consider using nf-schema plugin to validate inputs
- Use new smaller images in processes HOT 1
- Test use of smaller Docker images in workflow HOT 1
- Re-order bulk metadata to match order for overall sample metadata HOT 1
- Add sample metadata table to QC and cell type reports HOT 5
- Output bulk data to a `bulk` folder rather than individual files within project directory HOT 1
- Prepare for scpca-nf release `v0.8.2` HOT 4
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