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kurtwheeler avatar kurtwheeler commented on August 17, 2024

This image isn't the most clear, but this is the part of the pipeline this issue refers to.

salmon-gene-name

Further detail will be added later today.

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kurtwheeler avatar kurtwheeler commented on August 17, 2024

Ok, so it turns out that the github repo I linked to above pretty much says what needs to be done. salmontools extract-unmapped will expect an unmapped_reads.txt file output from Salmon and the original FASTQ file that was passed into Salmon. Therefore it will make sense to do this within the same Processor as the current Salmon processor.

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kurtwheeler avatar kurtwheeler commented on August 17, 2024

#84 answers the why of using STAR.

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jaclyn-taroni avatar jaclyn-taroni commented on August 17, 2024

After chatting with @dongbohu, here's a more up to date sketch

4_27_18 10_49 am office lens

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jaclyn-taroni avatar jaclyn-taroni commented on August 17, 2024

Chatted with @dongbohu this afternoon - the output of salmontools extract-unmapped appears to be a FASTA file. We think it makes sense to gzip it after the fact.

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dongbohu avatar dongbohu commented on August 17, 2024

Implemented in #250.

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