Comments (6)
This image isn't the most clear, but this is the part of the pipeline this issue refers to.
Further detail will be added later today.
from refinebio.
Ok, so it turns out that the github repo I linked to above pretty much says what needs to be done. salmontools extract-unmapped
will expect an unmapped_reads.txt
file output from Salmon and the original FASTQ file that was passed into Salmon. Therefore it will make sense to do this within the same Processor as the current Salmon processor.
from refinebio.
#84 answers the why of using STAR.
from refinebio.
After chatting with @dongbohu, here's a more up to date sketch
from refinebio.
Chatted with @dongbohu this afternoon - the output of salmontools extract-unmapped
appears to be a FASTA file. We think it makes sense to gzip it after the fact.
from refinebio.
Implemented in #250.
from refinebio.
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